The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Singlet Oxygen and Ultraweak Chemiluminescence in Rat Skin
WAKIO TORINUKITAKASHI MIURA
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1981 Volume 135 Issue 4 Pages 387-393

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Abstract

TORINUKI, W. and MIURA, T. Singlet Oxygen and Ultraweak Chemiluminemence in Rat Skin. Tohoku J. exp. Med., 1981, 135 (4), 387-393-The intensity of ultraweak chemiluminescence (CL) was estimated in rat epidermal homogenate with a technique utilizing UV irradiation (in vitro and in vivo). CL markedly increased in the homogenate after irradiation. The CL intensity was enhanced to 300% by the substitution of deuterium oxide for H2O, and it was reduced to 50% when diazabicyclo-octane, a scavenger for singlet oxygen, was added. It was con-cluded that the weak CL would be attributable to singlet oxygen, and the deter- mination of the lipoperoxide in skin tissue by the method of the CL counting system was discussed. singlet oxygen; ultraweak chemiluminescence; rat skin

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