The Minimum Effective Dose of Strychnine in Causing the Augmented Output of Epinephrine from the Suprarenal Gland in the Non=Anaesthetized, Non=Fastened Dog
1929 年 14 巻 2-3 号 p. 93-108
詳細
1929 年 14 巻 2-3 号 p. 93-108
The present investigation deals with the minimum effective dose of strychnine nitrate in causing an acceleration in the epinephrine liberation velocity in non-fastened, non-anaesthetized dogs. The blood of the suprarenal vein was collected extraperitoneally and the epinephrine was determined by means of the rabbit intestine strip.
0.01 mgrm. strychnine nitrate per kilo of body weight or 0.015 mgrm. was proved as the minimum effective amount. It coincides nearly with the minimum amount of the drug for evoking clinical symptoms, or a little smaller than the latter. The maximum surplus of the epinephrine secretion, evoked by the minimum effective dose of strychnine, was estimated as 0.00002-0.00006 mgrm. per kilo per minute, and the acceleration persisted for one hour or more.
When ether anaesthesia was resorted to, the method for collecting the suprarenal vein blood being that used in the experiments above discussed, the minimum effective dose of strychnine was estimated as 0.25 mgrm. per kilo of body weight. It is scarcely necessary to say that stress should not be laid upon the absolute amount of strychnine in the case of narcotised animals, for it is beyond doubt that the dose needed depends upon the depth of anaesthesia.
In two instances with non-anaesthetized dogs, in which an effective dose of strychnine, viz. 0.1 mgrm. per kilo, was administered, and the epinephrine was determined by means of the cat paradoxical pupil method of Sugawara, when at the accelerating influence of the drug upon the epinephrine secretion was disclosed. The only thing is that the absolute amount of epinephrine determinable by that method was somewhat inferior to that by the intestine method (Adrenalin chloride of Sankyo Co. was taken as the standard in carrying out the both kinds of determination). The average ratio of the values by both methods was 1:1.7 (Pupil: Intestine).
