Abstract
OBATA, M. Quantitative Studies on Sunburn Cell Formation in Mouse Epidermis. Tohoku J. exp. Med., 1983, 140 (4), 395-405 - Sunburn cell (SC) formation induced by single or combined doses of monochromatic ultraviolet-A (UV-A), ultraviolet-B (UV-B) and ultraviolet-C (UV-C) was studied quantitatively using mouse epidermal sheet preparations (NaBr split, H & E stain). Results were as follows: 1) Action spectra for SC formation showed that wavelengths shorter than 300nm were most effective. 2) A significant number of SCs was produced when a high dose of UV-A (360nm) was administered, and it was estimated that approximately 600 times more UV-A (360nm) energy was required to produce the same number of SCs as was required with UV-B (300 nm). 3) SC formation by UV-B (300nm) and UV-C (260nm) showed a logarithm-like dose-response relationship. 4) Time course studies showed that the maximum number of SCs was attained at 18 hr after UV-C (260nm) irradiation, at 24hr after UV-B (300nm) irradiation and at 30hr after UV-A (360nm) irradiation. 5) A high dose of UV-A (360nm) significantly increased the number of SCs induced by UV-B (300nm) or UV-C (260nm) when combined with them.
