Prostaglandin E₂ Production by Human Bone Marrow Cells: a Comparison with Peripheral Blood Mononuclear Cells

Abstract

NAKAYAMA, Y., SHINDO, T., AKIHAMA, T. and MIURA, A.B. Prostaglandin E₂ Production by Human Bone Marrow Cells: a Comparison with Peripheral Blood Mononuclear Cells. Tohoku J. exp. Med., 1984, 144 (4), 417-423 - The amount of prostaglandin E₂ (PGE₂) in the supernatant of human bone marrow cell cultures was measured by radioimmunoassay. Bone marrow cells were obtained from adult patients without hematological malignancies. Marrow mononuclear cells (BMMNC) (0.5×10⁶) and marrow nonadherent cells (BMNAC) (0.5×10⁶) were cultured at 37°C in 5% CO2 in 0.5ml RPMI 1640 supplemented with L-glutamine, 10% fetal calf serum and 10mM Hepes buffer. The levels of PGE₂ in the supernatant of BMMNC after 24 and 48hr incubation were 3, 000±2, 320pg/ml (n=10, mean±S.D.) and 4, 470±3, 510pg/ml (n=12), respectively. The kinetics of PGE₂ production by BMMNC was different from that of peripheral blood mononuclear cells. The level of PGE₂ in the supernatant of BMNAC after 48hr incubation was only 1/18 when compared to that of BMMNC. These data suggest that the major source of PGE₂ in the supernatant of marrow cell cultures may be bone marrow mononuclear phagocytes and that admixed blood mononuclear cells in BMMNC may produce only a small amount of PGE₂