Abstract
MIZUSHIMA, Y, IWATA, M., SATO, M. and YANO, S. Effects of Interleukin-2, OK-432 and Interferon-Gamma on In Vitro Induction of Nonspecific Killer Cells by Concanavalin A in Mice. Tohoku J. exp. Med., 1986, 148 (1), 79-85 -Human interleukin-2 (IL-2), OK-432 and mouse interferon-gamma (IFN-γ) were evaluated for their beneficial effects on in vitro induction of nonspecific killer cells by concanavalin A (Con A). Nonspecific killer cells could be induced by culturing C57BL/6 spleen cells for 4 days with Con A. Con A activated killer cells showed direct cell-mediated cytotoxicity (DCMC) and lectin-dependent cell-mediated cytotoxicity (LDCC) against natural killer (NK) sensitive YAC-1 cells, and LDCC against NK-resistant EL-4 cells in a 4hr-51Cr release assay. Higher DCMC and LDCC reactivity against YAC-1 cells, and higher LDCC reactivity against EL-4 cells were obtained with spleen cells cultured with Con A plus IL-2, or Con A plus OK-432 than with those cultured with Con A alone. No beneficial effect of IFN-γ and no additive effects of IL-2 plus OK-432, or IL-2 plus IFN-γ were observed in the induction of nonspecific killer cells by Con A.
