抄録
NAGAO, M., OYANAGI, K., TSUCHIYAMA, A., AOYAMA, T. and NAKAO, T. Studies on the Expression of Liver-Specific Functions of Human Fetal Hepatocytes in Primary Culture. Tohoku J. exp. Med., 1987, 152 (1), 23-29 - Biochemical functions of human livers were studied using fetal hepatocytes in primary culture. Immunocytochemical staining showed that albumin was not expresed in any fetal hepatocytes, whereas α-fetoprotein was detected in almost all the cells. Tryptophan 2, 3-dioxygenase (TO, EC 1.13.11.11.) activity was not induced in the presence of 10-7M dexamethasone and 10-7M glucagon, but the activity of tyrosine aminotransferase (TAT, EC 2.6.1.5.) was elevated about 35 fold under the same conditions. These results suggest that the TAT and α-fetoprotein genes are activated in human fetal liver at 14 to 20 weeks of gestation.
