抄録
OYANAGI, K., KUNIYA, Y., NAGAO, M., TSUCHIYAMA, A. and NAKAO, T. Cytotoxicities of Sodium Benzoate in Primary Culture of Hepatocytes from Adult Rat Liver. Tohoku J. exp. Med., 1987, 152 (1), 47-51 - The cytotoxicities of sodium benzoate was studied using primary culture of hepatocytes established from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in serum-free culture medium. The activities of ornithine transcarbamylase (as a marker of mitochondria) and tyrosine aminotransferase (as a marker of cytosol) were clearly suppressed by sodium benzoate at concentration in excess of 500μg/ml. Intracellular protein synthesis and DNA synthesis were also suppressed, and the suppression of DNA synthesis was observed even with a lower concentration of benzoate (100μg/ml).
