Chronic Granulomatous Disease with Neutrophil Membrane Cytochrome b Deficiency: Demonstration by Immunochemical Staining with Monoclonal Antibody

Abstract

MINEGISHI, N., NAKAMURA, M., SUZAKI, K., TERASAWA, M., MINEGISHI, M., TSUCHIYA, S. and KONNO, T. Chronic Granulomatous Disease with Neutrophil Membrane Cytochrome b Deficiency: Demonstration by Immunochemical Staining with Monoclonal Antibody. Tohoku J. exp. Med., 1988, 154 (2), 143-148 -Cytochrome b deficicency in the peripheral granulocytes of two male patients with chronic granulomatous disease was demonstrated by an immunocytochemical assay using a monoclonal antibody, 7D5, against human neutrophil cytochrome b. A mosaic of cytochrome b positive and negative neutrophils, indicating a carrier state in an X-linked trait, was found in the mother of patient 1 but not in the mother of patient 2.