抄録
KIKUCHI, H., SEKIYA, T., NISHIMURA, S. and WATANABE, M. Repetitive Sequences in the Rat Genome that Have Cleavage Sites for the Restriction Endonuclease Taq I. Tohoku J. Exp. Med., 1990, 161 (2), 91-99-A cleavage of rat DNA with restriction endonuclease Tag I produced at least 8 discrete bands. One of the bands, 0.29 kilobase pairs (kb) long, was isolated and cloned in plasmid. The hybridization of this fragment to genomic DNA digested with Taq I showed 1.6kb, 1.2kb and 0.29kb band in a smear hybridization signal. A repetition of the DNA sequence of this fragment was about 12, 500 copies haploid genome of both Buffalo and Sprague-Dawley rats by the Cot-hybridization analysis. Four independent clones of 0.29kb Taq I fragment share high homology (97%).
