Abstract
AOSHIBA, K., NAGAI, A. and TAKIZAWA, T. Effects of Proteinase Inhibitors on Polymorphonuclear Neutrophil Polarization. Tohoku J. Exp. Med., 1991, 165 (3), 165-170-Polarization of polymorphonuclear neutrophils (PMNs) can be elicited by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) and the microtuble-disrupting compound colchicine. Here we report on whether natural and synthetic proteinase inhibitors alter the polarizing response to these two agents. The α1-proteinase inhibitor, N-tosyl-L-phenylalanine chloromethyl ketone, and Nα-tosyl-L-lysine chloromethyl ketone suppress fMLP-induced polarization and locomotion in a dose-dependent fashion, but none of them affects colchicine-induced polarization. We suggest that proteinase inhibitors suppress fMLP-induced polarization by blocking cell surface proteinases that generate an intracellular signal for cytoskeletal change and polarization.
