抄録
ZAVOS, P.M., SDFIKITIS, N., TODA, T. and MIYAGAWA, I. Improvements in Qualitative Characteristics of Cryopreserved Human Spermatozoa Following Recovery via the Use of the SpermPrepTMII Filtration Method. Tohoku J. Exp. Med., 1991, 165 (4), 283-290-Motile, morphologically normal human spermatozoa can be separated from semen via a disposable SpermPrepTM filtration method. This method was employed with great success using frozen-thawed spermatozoa. The present study examined the qualitative characteristics of filtered spermatozoa via a new SpermPrepTMII filtration method in conjunction with the short-term freeze preservation of these spermatozoa. The isolation procedure yielded populations of spermatozoa with very high percentage motility and progressive motility grade (0-4) and which were free of seminal debris. For 20 semen samples the prefreeze values of percentage of motility and grade for the fresh and post-filtered were 58.6% and 3.1 vs 84.1% and 3.6, respectively. The sperm freeze preservation procedure involved dilution of the spermatozoa in Test-Yolk buffer (TYB) with 7% glycerol (v/v) and freezing and thawing in a conventional manner. Post-thaw percent motility, and survival % were substantially higher (p<0.05) for the filtered fraction than for the parent semen. The filtered fractions yielded postthaw mean values of motility percent and grade of 68.9 and 3.4 vs 47.6 and 3.0 for the parent semen, respectively. Survival following incubation at 37°C for 3 hours yielded respective values of 75.9% and 53.2% (p<0.05). It was concluded that the major factor in improving post-thaw quality recovery overall was the filtration via the SpermPrepTMII as applied in this study. This technique could have significant clinical applications in the use of frozen-thawed specimens for noncoital reproduction purposes.
