Possible Involvement of Nuclear Oncoproteins in Regulation of DNA Replication

Abstract

ASANO, M., ISHIKAWA, H. and ITO, Y. Possible Involvement of Nuclear Oncoproteins in Regulation of DNA Replication. Tohoku J. Exp. Med., 1992, 168 (2), 183-187-Polyomavirus DNA replication requires its enhancer which contains an AP-1 site. We have shown that protooncogenes, c-jun and c-fos, whose products form heterodimeric transcription activator, AP-1, strongly stimulate polyomavirus DNA replication through the AP-1 site. The mechanisms by which this enhancer stimulates replication and transcription are different. By replacing the enhancer with the oligonucleotides representing the binding site of a transcription factor of interest, any transcription factor with the known binding sequence can be characterized in this replication assay. When Rel protein was examined, we were able to reveal a new domain in v-Rel protein which can stimulate replication strongly. Whether this domain also coincides with transforming potential of v-Rel protein is currently under investigation.-c-fos; c-jun; DNA replication; polyomavirus; Rel