Abstract
ASANO, M., ISHIKAWA, H. and ITO, Y. Possible Involvement of Nuclear Oncoproteins in Regulation of DNA Replication. Tohoku J. Exp. Med., 1992, 168 (2), 183-187-Polyomavirus DNA replication requires its enhancer which contains an AP-1 site. We have shown that protooncogenes, c-jun and c-fos, whose products form heterodimeric transcription activator, AP-1, strongly stimulate polyomavirus DNA replication through the AP-1 site. The mechanisms by which this enhancer stimulates replication and transcription are different. By replacing the enhancer with the oligonucleotides representing the binding site of a transcription factor of interest, any transcription factor with the known binding sequence can be characterized in this replication assay. When Rel protein was examined, we were able to reveal a new domain in v-Rel protein which can stimulate replication strongly. Whether this domain also coincides with transforming potential of v-Rel protein is currently under investigation.-c-fos; c-jun; DNA replication; polyomavirus; Rel
