Abstract
OHASHI, Y., MINEGISHI, M., TSUCHIYA, S. and KONNO, T. Three Monoclonal Antibodies against Human LEA-1 α and β Chains with Different Biological Activities. Tohoku J. Exp. Med., 1992, 168 (4), 599-610-Three murine monoclonal antibodies (MAbs) directed against human lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18), designated as MAY.017, MAY. 035, and MAY. 044, were newly generated. The hybridomas were screened for their ability to inhibit a phorbol ester-stimulated aggregation of Epstein-Barr virus-transformed B-lymphoblastoid cell line (B-LCL). The MAbs bound to peripheral blood leukocytes, T cell lines, B cell lines, and some of myeloid/monocytic lines, but not to B-LCL derived from a patient with leukocyte adhesion deficiency. MAY.035 immunoprecipitated a complex of proteins with molecular masses of 155kDa and 95kDa, while MAY.017 and MAY.044 did a complex of 130kDa, 155kDa and 95 kDa proteins. MAY.035 was shown as to recognize the α chain of LFA-1(CD11a), and both MAY.017 and MAY.044 the β chain of the β2 integrin family (CD18). All the three MAbs inhibited lymphocyte proliferative responses to mitogens or alloantigens. MAY.017 blocked cytolytic activity mediated by natural killer cells. -monoclonal antibody; integrins; lymphocyte function-associated antigen-1; leukocyte; bone marrow transplantation
