Effects of Percoll Discontinuous Density Gradients vs SpermPrep^TMII* vs Sephadex G-50 Gel Filtration on Semen Parameters.

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CHECK, J.H., ZAVOS, P.M., KATSOFF, D., KIEFER, D. Effects of Percoll Discontinous Density Gradients vs SpermPrep^TMII* vs Sephadex G-50 Gel Filtration on Semen Parameters. Tohoku J. Exp. Med., 1993, 169 (3), 225-231- There are various methods of separating sperm from seminal plasma for subsequent intrauterine insemination (IUI) and for in vitro fertilization (IVF). The purpose of the present study was to assess and compare semen parameters following Sephadex, Percoll and SpermPrep^TMII separation techniques. The SpermPrep^TM II is also a Sephadex preparation but uses a different bead size, less Sephadex and is a quicker method. The specimens (n=16) were initially evaluated for count, (C; ×10⁶/ml) % motility (MO), grade of motility (GR; %), and HOS test scores. Each specimen was then divided into 3 equal aliquots and prepared as follows: 1) layered onto a modified Percoll discontinuous density gradient column; 2) processed using SpermPrep^TMII; and 3) filtered through a Sephadex gel filtration column (G-50). The results show all 3 treatments yielded lower (p<0.01) counts as compared to control values but no differences were noted among them. Percoll and SpermPrep^TMII increased MO (p<0.01) where Sephadex reduced it (p=NS). Percoll and SpermPrep^TMII improved the % of best quality sperm but not Sephadex with SpermPrep^TMII being higher than Percoll. There were increases (p< 0.05) in HOS values in all experimental treatments with SpermPrep^TMII being the best. However, this study did not show as many males with HOS scores below 50% as noted in other studies. Finally, Percoll and SpermPrep^TMII seem equally effective methods for producing high quality sperm for IUI or IVF although SpermPrep^TMII is quite faster.- SpermPrep^TMII; Sephadex; Percoll discontinuous density gradient; hypoosmotic swelling test; semen filtration