The Effect of the Removal of the Suprarenals and Accessory Cortical Tissues Together upon Some Blood Constituents of Rabbits

抄録

In male rabbits the one suprarenal gland was removed together with the accessory cortical tissues in a three stage operation, and after recovery the blood constituents were determined: the total nitrogen, non-protein nitrogen, urea nitrogen, the preformed and total creatinine, uric acid, chlor, inorganic phosphorus (in serum), sugar, haemoglobin and water, and again after recovery the second suprarenal gland was extirpated, and either in still apparently normal condition and in a weakened or even a moribund state blood was drawn out, and followed by analysis.
When tested in the incomplete cortical tissue deflciency, either before extirpating the second suprarenal gland or when accessory cortical mass was left behind after extirpation, the blood constituents were found wholly normal.
In the rabbits suffering from complete cortical tissue deflciency the following changes were found as occurring:
(1) Haemoglobin content increased, but it was not very considerable, the maximum being found as 24 per cent of the initial. It is not likely to increase rapidly at the terminal stage.
(2) Water content diminished invariably, but slightly.
It is doubtless that the blood is concentrated in the rabbit in the complete cortical tissue deflciency, but its degree was by no means significant, contrary to the view of some writers who made use of other kinds of animals.
(3) Blood sugar was found decreased in the enfeebled state of rabbits suffering from the deficiency.
(4) Total nitrogen and protein nitrogen increased in the cortical tissue deficiency. When the rabbits were apparently still more or less healthy the increase was not large.
(5) Non-protein nitrogen increased, and its degree of increase was distinctly greater than that of the total nitrogen (and protein nitrogen), and when the animal was already weak, the increase was also large, generally speaking.
(6) Urea nitrogen increased in the complete suprarenocortical deficiency in about the same ratio with the non-protein N, but in the cases of urea N there does not exist any definite relation between the clinical symptoms and the magnitude of increase of urea N.
(7) Preformed and total nitrogen increased invariably, and its degree more or less depended upon the weakness of the animals, roughly speaking.
(8) Uric acid increased also, but the intensity of increase finds some similarity to that of urea N in relation to the clinical symptoms.
(9) Chlor decreased, and its magnitude depended apparently on the interval allowed to elapse after the complete removal of all the cortical tissues till to the blood collection.
(10) Serum inorganic phosphorus increased in the deficiency and the increase was greater when the animals were weak.
Thus in the suprarenocortical deficiency, of the blood constituents the total N, protein N, non-protein N, urea N, preformed and total creatinine, uricacid, and serum inorganic phosphorus increased in concentration, and the chorides and sugar diminished. The haemoglobin content increased and the water reduced. Of these the ratio of increase and decrease respectively to the initial value was found materially wholly or nearly same in the nonprotein N, urea N, serum inorganic phosphorus and chlor. And there was further detectable some relationship, though not quite so definite, between the severity of the clinical symptoms and the magnitude of increase in the concentration of the non-protein N, creatinine and serum inorganic phosphours, while it was difficult to find such a relation for urea N and uric acid. Between the latter two exists seemingly somewhat of a parallelism. The magnitude of increase of chlor depended chiefly upon the length of time elapsing after the beginning of the complete deficiency.