Abstract
SHAMSA, F., IWASA, T., SAITO, H., NAGATA, N. and OHTSUKI, K. The Biological Significance of Glycyrrhizin- and Glycyrrhetinic Acid Derivative-Induced Selective Phosphorylation of Histones H2A and H2B by A-kinase In Vitro. Tohoku J. Exp. Med., 1994, 172 (2), 123-132 - Histones H2A and H2B were found to be glycyrrhizin (GL)-binding proteins, because (i) the two histones H2A-H2B pairs were isolated selectively from the crude histone preparations of calf thymus by means of GL-affinity column chromatography (HPLC); (ii) phosphorylation of these two histones by A-kinase was remarkably stimulated by native GL or oGA (a derivative of glycyrrhetinic acid) at 20μM; and (iii) in the crude histone preparations of calf thymus, these two histones were selectively phosphorylated by A-kinase in the presence of both dsDNA and 20μM oGA or 20μM GL. The provided data suggest that the GL-induced selective phosphorylation of histones H2A and H2B by A-kinase may be implicated in the transcriptional activation involved in the biological activities of the drug.
