The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Establishment of a Cell Line from an Adenocarcinoma of the Lung Producing Carcinoembryonic Antigen (CEA) and CA19-9
MAKOTO MIKI, TOYOHARU ISAWA, TAKEO TESHIMA, YOSHIKI ANAZAWA, ASIF MUJTABA MAHMUD, TOSHIHIRO NUKIWA
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キーワード: primary culture, lung cancer, characterization, CEA and CA19-9
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1995 年 175 巻 4 号 p. 269-278

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MIKI, M., ISAWA, T., TESHIMA, T., ANAZAWA, Y., MAHMUD, A.M. and NUKIWA, T. Establishment of a Cell Line from an Adenocarcinoma of the Lung Producing Carcinoembryonic Antigen (CEA) and CAJ9-9. Tohoku J. Exp. Med., 1995, 175 (4), 269-278 - An adenocarcinoma cell line was established from pleural effusion of a 67-year-old woman and designated as TK-SA. The population doubling time of the cells was 100.4hr. Chromosomal analysis revealed the TK-SA to have human aneuploid chromosomes with a near-triploid mode. Ultrastructurally, the TK-SA was compatible with adenocarcinoma. The cell line was highly tumorigenic. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide dye assay demonstrated resistance of the cell line to Cis-platin (CDDP), Cyclophosphamide (CPM) and Tegafur/Uracil (UFT). High carcinoembryonic antigen (CEA) and CA19-9 levels were similar in patient's serum and conditioned medium.
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    The Tohoku Journal of Experimental Medicine (TJEM) was founded in 1920 by professors of Tohoku Imperial University, Medical School. The TJEM has been published continuously, except for the year of 1946 just after the World War II. The TJEM is open to original articles in all branches of medical sciences. The TJEM also covers the fields of disaster-prevention science, including earthquake archeology.


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