Abstract
NAGAE, M., AOKI, Y., NATA, M., HASHIYADA, M. and SAGISAKA, K. Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application. Tohoku J. Exp. Med., 1995, 177 (4), 353-364 - A mouse monoclonal antibody against an amniotic protein carrying ABH antigenic epitopes was established. BALB/c mice were immunized by an amniotic protein of molecular weight over 200kDa, which had proved to be the carrier protein of ABH blood group epitopes by analysis with SDS-PAGE and immunoblotting. The antibody, ASP-1, was directed to the amniotic carrier protein without affecting the ABH blood group antigenicity, and did not cross-react with other body fluids which included blood, saliva, semen, urine or vaginal secretion. The immunoglobulin class of ASP-1 was IgG1 with a titer of 1:1, 600. ASP-1 was used to detect the ABH blood group of amniotic fluid by the sandwich ELISA in which wells of plates were coated with ASP-1, and the ABH blood group of the captured protein was detected with mouse IgM anti-A and -B antibodies and enzyme conjugated anti-mouse IgM. The sandwich ELISA could successfully detect the blood group of amniotic fluid in mixed body fluids. - amniotic fluid; ABH blood group; ELISA; monoclonal antibody; forensic serology
