抄録
The normal shortest peroxidase stain-time of blood neutrophiles (from non-B-avitaminotic individuals) is universally two second (for Solution II of Sato-Sekiya's reagents). That from a B-avitaminotic body shows a prolongation of 1-8 seconds. But even normal blood neutrophiles show a prolongation, if the blood film is treated with an etherous milk-extract of a B-avitaminotic mother or of a mother with negative Arakawa's reaction. The result is the same, if methyl glyoxal is used instead of the milk extract.4) And a methyl glyoxal like substance is extracted from Arakawa-negative milk.
In the present work, the urine has been made the object of investigation. If the above statement is true, then one will think that the normal shortest peroxidase stain-time may be prolonged by a pretreatment of normal blood neutrophiles with urines from a B-avitaminotic body or from a mother with Arakawa-negative milk. And the actual result has shown us that the urines of mothers with Arakawapositive milk did not cause any prolongation of the normal shortest peroxidase stain-time of normal blood neutrophiles, while those of mothers with Arakawa-negative milk very often caused a prolongation in different grades. As Geiger and Rosenberg identified methyl glyoxal from the urines of B-avitaminotic bodies in clinical and experimental medicine, then it is probable that mothers with Arakawa-positive milk do not excrete urine containing methyl glyoxal at least in a noticeable amount, whereas it is highly probable that mothers with Arakawa-negative milk excrete methyl glyoxal in their urines in a remarkable amount.
It is however to be noted here that the fact that the urine of a lactant has no prolonging effect in question will not exclude necessarily that she is utterly free from avitaminosis B, because the prolonging effect of a urine seems to be a less sensitive test than Arakawa's reaction.
