Abstract
Effect of hydrogen ion concentration, MgCl2 and phosphate on the tissue respiration of rabbit kidney cortex was investigated by Warburg's second method.
(1) Three kinds of Ringer's solution of pH 7.02, 7.20 and 7.42 were prepared by varying the bicarbonate concentration of the solution against the definite concentration of CO2 gas. The tissue respiration in these various solutions was compared with that in the normalRinger's solution of pH 7.32, and it was found that no marked dif-ference was caused by the variation of pH in these ranges.
(2) Ringer's solutions of three kinds of MgCl2 concentration, i.e., 1, 5 and 10 mgm per cent were prepared and the tissue respiration was measured against that in the normal Ringer's solution.
It was proved that MgCl2 seemed to be indifferent to the tissue respiration in vitro at such concentrations.
(3) 45, 90 and 180 mgm per cent phosphate was added to 100cc Ringer's solution, and the tissue respiration was estimated by comparing with that in the normal Ringer's solution.
The tissue respiration was increased with the increase of phos-phate in the Ringer's solution.
