Arakawa's Reaction and Vitamin C Content of Human Milk

Part 5. Difference between Arakawa-negative Human Milk and Human Milk made Arakawa-negative by Ascorbic Acid added. 123rd Report of the Peroxidase Reaction

Abstract

I investigated the oxidation of the vitamin C like substance in human milk and that of the ascorbic acid by means of the Arakawa Reagent III and knew that peroxide and guaiacol (or guaiacol-like substance) are necessary for this oxidation. Further, I knew that an addition of the reagent can oxidize a large amount of ascorbic acid, and was able to differentiate between human milk with AR 5' (-)^* and Arakawa-positive milk made Arakawa-negative by ascorbic acid.