Short=timed Peroxidase Reaction of Blood Leucocytes of B₁=avitaminotic Rats; and Effect of Different Amounts of Vitamin B₁ on It

163rd Report of the Peroxidase Reaction. (110th Human Milk Study.)

Abstract

1. A prolongation of the shortest peroxidase stain-time is also seen in B₁-avitaminotic rats.
2. The longer the B₁-avitaminotic feeding, the more pronounced the prolongation becomes. It maybe prolonged to 9-10 seconds in the terminal stage.
3. A daily administration of 0.03 mgrms. of B₁-crystals restored the prolonged stain-time to normal in 3 days averagely, that of 0.01 mgrm. in 5 days, and that of 0.005 mgrms. in 7 days. A prolongation was restored to normal in time longer than 7 days in the rats which had survived on a daily administration of 0.001 mgrm. of B₁-crystals, while the prolongation was not restored to normal in the rats which had died in spite of an administration of the same dose. In the last series of experiment in which 0.0005 mgrms. of B₁-crystals were used, the prolongation was restored at a much slower pace, in order to re-gain its former figures or even larger figures. At length they died in 5 days averagely without exception.