The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
The Salt Error of Brom-thymol-blue
A Note on the Colorimetric Determination of pH with Brom-thymol-blue at 38°C
Kojiro MatsudaYoshishichiro Koyama
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1949 Volume 50 Issue 3-4 Pages 235-239

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Abstract
The apparent ionization exponent pK'2 of B. T. B. at 38°C was deter-mined in phosphate mixtures and solutions of sodium chloride of various ionic strength up to 0.133 Mol/l in phosphate mixtures and 0.308 Mol/l in sodium chloride solutions. The thermodynamic ionization exponent pK2 obtained by extrapo-lation to μ→O is 7.33 at 38°C.
pK'2 values thus obtained make possible to eliminate the “salt error” of. B. T. B. and can be profitably, used, for pH determination of biological fluids at body temperature.
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© Tohoku University Medical Press
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