The Salt Error of Brom-thymol-blue
A Note on the Colorimetric Determination of pH with Brom-thymol-blue at 38°C
1949 年 50 巻 3-4 号 p. 235-239
詳細
1949 年 50 巻 3-4 号 p. 235-239
The apparent ionization exponent pK'2 of B. T. B. at 38°C was deter-mined in phosphate mixtures and solutions of sodium chloride of various ionic strength up to 0.133 Mol/l in phosphate mixtures and 0.308 Mol/l in sodium chloride solutions. The thermodynamic ionization exponent pK2 obtained by extrapo-lation to μ→O is 7.33 at 38°C.
pK'2 values thus obtained make possible to eliminate the “salt error” of. B. T. B. and can be profitably, used, for pH determination of biological fluids at body temperature.
