抄録
1. A method for estimating free acetylhexosamine or bound acetylhexosamine with bare CI was devised.
2. An enzyme mixture of snail liver (called ‘the original enzyme solution’) could not split acetyl from N-acetylglucosaminides.
3. Optimum pH's for α- and β-acetylglucosaminidase in it were found to be 4.5.
4. β-Acetylglucosaminidase could be separated from α-acetylglucosaminidase by adsorption of the latter to aluminium silicate. A β-acetylglucosaminidase solution, thus prepared, contained also β-galactosidase.
5. The original enzyme solution as well as the β-acetylglucosaminidase solution above could hydrolyze hardly the group carbohydrate from pig-stomach mucus but comparatively easily a partial-scission product of that carbohydrate with increase of the amount of free acetylglucosamine.
6. From the findings in 5. a conclusion was drawn that acetylglucosamine molecules in the inner part of the group carbohydrate chain take β-glucosidic type.
