Nucleic Acids
VIII. Chemical Comparison between DNAs of Human Placenta and of Human Spleen First Report: On Composition and Physical Properties
1957 年 65 巻 2-3 号 p. 269-279
詳細
1957 年 65 巻 2-3 号 p. 269-279
1. Deoxyribonucleic acids were isolated as sodium salts from human spleen and placenta in a state as pure and highly polymerized as possible, and compared with each other.
Fig. 3. Relative viscosities of the watery solutions of the splenic DNA (Prep. 4) (_??_) and of the placental DNA (Prep. 5') (_??_). Temperature 25°C
2. Although the splenic DNA preparations were mostly not contaminated with more than 1% pentosenucleic acid, those from placentas contained even 4-5% pentosenucleic acid.
3. The DNAs resemble each other in composition.
4. Although they gave similar values of ε(P)max in water which show poor reproducibility, the placental-DNA preparations gave definitely lower ε(P)max values than the splenic-DNA preparations in 0.01N NaOH as against the corresponding values of the respective DNA preparations in water.
5. The both DNAs became more absorptive when heated in water at 70, 80 and 90°C. The higher the temperature of heating was, the higher was elevation of the absorptivities. The placental DNA showed the same elevation at 90 and 100°C contrary to the splenic DNA, which
Fig. 4. Skin reaction of the splenic DNA and placental DNA.
became still more absorptive at the latter temperature than at the former.
6. The placental DNA gave less viscous solutions than the splenic DNA. The findings in 4. to 6. above suggest a lower polymerization grade of the placental DNA than that of the splenic DNA.
7. The placental DNA is less dextrorotatory than the splenic DNA. 8. The placental DNA shows more intensive skin reaction in cancer patients than the splenic.
Thanks are due to the Ministry of Health and Welfare for a grant in aid to us, which is gratefully acknowledged. H. Masamune.
