Abstract
A serum-free culture medium was devised which supports the growth of L line cell subcultured in a serum containing usual medium and enables it to propagate from its low density of inoculum. This serum-free medium consists of Eagle's minimum essential medium supplemented with bovine albumin, insulin, serine, pyruvate, protamine (or FeCl3) and lactalbumin hydrolysate in replacement of serum. This medium supported the cellular growth from such low density of the inoculum as that employed in “single cell culture technique” and colony forma-tion was observed with the above technique.
