Abstract
The micromethod technique for the determination of enzyme activities in liver is out lined.
It is demonstrated that the micromethod technique is reliable.
Glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, alkaline and acid phosphatases, glucose-6-phosphataso, catalase, aldolase and arginase activities in liver and serum were measured in various liver and malignant diseases.
Routine hepatic function tests were done simultaneously and the results were compared with the results of hepatic enzyme studies. This study showed significant correlation existing only between alkaline phosphatase activity in liver and A/G ratio. Simultaneously done histological studies demonstrated significant correlation existing only between the alkaline phosphatase activity in liver and the grade of fibrosis in the liver. The duration of the hepatic disease correlated significantly with glutamic oxaloacetic transaminase and glutamic pyruvic transaminase activities in the liver.
Although the changes in enzyme activities of the serum and the liver in majority of the cases showed concomitant and symmetrical pattern of the changes, enougn number of cases deviated from this general rule, suggesting the necessity of further study in regard to the mechanism of the increase of these enzyme activities in the serum, mode of activation of the liver enzymes, its turnover, in short, the dynamic mechanism of the liver enzymes.
