Quantitative Determination of Calcium Bilirubinate in Gallstone by Infrared Spectroscopy

抄録

Attempts were made at a simple, rapid and precise determination of calcium bilirubinate in the gallstone by means of infrared spectroscopy. The KBr-disk method was adopted for this purpose, the absorbance being calculated according to the base-line method. Of a number of absorptions characteristic of calcium bilirubinate, the 1624cm^-1 band was determined to be most suitable for the key band of this compound in view of its satisfactory intensity, perfect adherence to Beer-Lambert's law and little susceptivity to interference of coexisting substances. A calibration curve was established at this wave number using authentic calcium bilirubinate, which proved to be valid also for multi-component samples simulating gallstones. The concentration of calcium bilirubinate in gallstone specimens as determined by this method fell in between analytical data obtained by two chemical methods, assuring the accuracy of this spectroscopic method.