A Simple Thin-layer-chromatographic Method for Detection of Urinary Homocystine
1967 年 91 巻 1 号 p. 31-34
詳細
1967 年 91 巻 1 号 p. 31-34
For the detection of homocystine in urine specimens, a simple thin-layer chromatographic method was devised. Thirty μ1 of urine specimens without any prior treatment were applied onto a silica gel-G plate, and developed by ascending chromatography using a phenol water mixture (3:1) containing 0.05% potassium cyanide. After developing and drying the plate, a slightly modified potassium iodoplatinate reagent was sprayed. Homocystine, cystine, methionine, cystathionine and histidine were clearly separated with different Rf values of 23-27, 12-13, 59-64, 14-16 and 44-4, respectively.
