Abstract
A technique for measuring plasma angiotensinase of the rabbit is described. A mixture composed of 2.0ml of 1/15 AI phosphate buffer, 0.5ml of plasma, 0.5ml of saline and 1.0ml of saline containing 1μg of angiotensin is incubated at 37°C for 10 minutes and residual angiotensin is determined by the bracket technique on the rat blood pressure preparation. The plasma angiotensinase activity is indicated by percentage of inactivated angiotensin. Plasma angioten-sinase activity of 20 normal rabbits was 55.6±7%. It remained within normal limits in 5 renal hypertensive rabbits until the 40th week of operative renoarterial constriction.
