Abstract
A simple alcian blue method was presented for identifying individual acid mucopolysaccharides in cryostat sections. This method was based on the preci-pitation of acid mucopolysaccharides with alcian blue and the subsequent differentiation of individual acid mucopolysaccharides with MgCl2 solution at a critical salt concentration. In spot test and tissue sections, the present method differentiated successfully between hyaluronic acid, chondroitin sulfate and heparin.