The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
A Simple Method for Histochemical Identification of Acid Mucopolysaccharides in Cryostat Sections
Yoichiro SasaiHiroshi Akiba
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1968 Volume 94 Issue 4 Pages 385-391

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Abstract
A simple alcian blue method was presented for identifying individual acid mucopolysaccharides in cryostat sections. This method was based on the preci-pitation of acid mucopolysaccharides with alcian blue and the subsequent differentiation of individual acid mucopolysaccharides with MgCl2 solution at a critical salt concentration. In spot test and tissue sections, the present method differentiated successfully between hyaluronic acid, chondroitin sulfate and heparin.
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© Tohoku University Medical Press
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