Inhibitory Effect of Methylenedianiline on Testicular 17-Oxosteroid Secretion Produced by Pregnant Mare Serum

Abstract

The effect of methylenedianiline on testicular 17-oxosteroid secretion caused by pregnant mare serum (PMS) was evaluated by measurements of 17-oxosteroids in the spermatic vein blood of the dog. Testicular 17-oxosteroid secretion in dogs which received a single injection of PMS (2, 000 per animal) into the saphenous vein increased markedly and reached the maximum within approximately 90 minutes after the injection; the increase in secretion tended to be maintain-ed over 4 hours of observation. At the highest rate of secretion, an intravenous injection of methylenedianiline (20 or 60mg/kg) into these dogs brought about a rapid decline in the output of testicular 17-oxosteroids. The response was rever-sible. These results show that methylenedianiline can prevent the augmented secretion of testicular 17-oxosteroids caused by pregnant mare serum.