抄録
The cell spheroid having high differentiation ability is biologically interesting as a promising technology for tissue engineering. In the present study, we've succeeded in controlling the size of cell spheroids which were two-dimensionally aligned on the micropatterned substrate. To use the spheroids for tissue engineering as an implantable construct, we designed the biocompatible hydrogels with photo-crosslinkable polymer, based on end-acrylated multi-armed poly(ethylene glycol). These gels' storage elastic modulus G' was determined from rheologycal test. As a result, the gels' modulus covered between 100 and 2500Pa by changing the different concentrations and types of macromers. The permeability of these hydrogels was determined using the two-chamber cells. In use of sodium benzoate as probe molecules, the probe passed through all the gels, while no clear effect in the different types of monomers was observed. After photoencapsulation of the chondrocyte spheroids, the multi-array formation of spheroids were maintained in the hydrogel. Biochemical analysis demonstrated that aggrecan increased for 1 week on the hydroge1s and the viability of the cell. Therefore, noble cell spheroid sheet was established using PEG hydrogel. These findings suggest the gel may have high utility as an implant multi-arrayed cell spheroids.
