抄録
Biocompatibility of silicone rubber sheet (SR) was improved by the carbon negative-ion implantation for the attachment of mesenchymal stem cells (MSC). The carbon negative ions were implanted at 10 keV and various ion doses from 0.01 - 10 (x10^15) ions/cm^2 through the micropattern mask with aperture slits of 50-micro m width for study of the selective attachment property. Contact angles of the modified SR at the same conditions without the micro-pattern mask were measured by water drop and air bubble. After 25-day in vitro culture of MSC from rat bone marrow on the implanted SR, the phase contrast micrographs showed the attachment of cells only on the stripe regions implanted with doses from 1 - 10 (x10^15) ions/cm^2. The good conditions for cell attachment are 3 and 10 (x10^15) ions/cm^2 of ion doses corresponding to the low contact angle, which is 69° and 61°. The fluorescence micrographs also confirmed that the position of cell nucleus and the actin filament on the implanted region at these implantation conditions. As implanted at low dose, no cell attached and cells very weakly attached.
