抄録
The adsorption properties of proteins such as laminin (LN), fibronectin (FN) and gelatin on the carbon negative-ion implanted silicone rubber sheet (SR) including their effects of these protein-coated modified SR on patterning nerve-cell culture were investigated. The implantation conditions were fixed at 3x10^15 ions/cm^2 and 10 keV. The concentrations of LN in PBS, FN in PBS and gelatin in de-ionized water (DIW) were 0.5-50, 0.5-10 and 5-10^3 micro g/ml, respectively. The adsorption properties of proteins on SR sheet implanted as a half moon shape were evaluated by XPS. After 4-day in vitro culture of the nerve-like cell of rat adrenal pheochromocytoma (PC12h) on the protein-coated surfaces of SR implanted through the micro-pattern mask with slits of 50-f..l,m width, the phase contrast micrographs show that the suitable protein for patterning the attachment of nerve cell was FN at 1 micro g/ml of concentration, corresponding to high ratio of nitrogen adsorption between implanted and unimplanted regions that was 1.9. As coated with 1 micro g/ml of LN, cells attached over all areas. While coating with 1 mg/ml of gelatin, no cell attached.
