抄録
Microgels composed of poly(N-isopropylacrylamide-co-acrylic acid) with different acrylic acid contents were prepared. The dispersion of microgels with higher AAc content exhibited affinity with human immunoglobulin G (IgG). The maximum adsorption was observed around pH 5.0, while both low pH and basic conditions induced the rather low adsorption caused by electrostatic repulsion. Two-dimensional (2D) arrayed microgel was prepared and IgG adsorption on the an-ay was compared with above mentioned adsorption in dispersion of microgels. Adsorptions of IgG on 2D particle array and in the dispersion exhibited similar dependence on both pH and temperature. Surface plasmon resonance (SPR) was used to investigate the kinetic parameters of protein binding onto 2D-arrayed microgels. The association and dissociation rate constants and the corresponding affinity at various pHs were obtained.
