抄録
To solubilize pachyman (beta-1,3 glucan) in water, the application of macchinetta extractors was investigated. Superheated water of about 140oC was generated in the high pressure produced by a pressure building bed constructed of tightly compressed powder of pachyman itself The superheated water caused hydrolysis of pachyman while passing through the packed bed. The oligosaccharides produced by partial hydrolysis were immediately extracted from the macchinetta without hard damage such as pyrolysis. The extraction was sufficient within 5 min. The highest extract yield was 5.5% of the raw material and 11 times that from a Soxhlet extractor. The yields increased with an increase of water temperature, which was controllable by varying the packed bed thickness. Furthermore, using moist powder was a practical improvement of the pressure building ability of a packed bed. The extracts obtained were 21% glucose, 4% disaccharide and 75% oligomer. The average molecular weight was 1.6-1.9 x l03, which corresponds to 10-12 degrees of polymerization. In addition, it was confirmed that the elution had bioactivity to the special enzyme extracted from the blood cells of a horseshoe crab.
