Correlation between Prostatic Atrophy and Apoptosis in the Canine Spontaneous Benign Prostatic Hyperplasia (BPH) Following Chlormadinone Acetate (CMA) Administration

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In order to confirm the relationship between benign prostatic hyperplasia (BPH) regression and atrophic effect of chlormadinone acetate (CMA), the prostates of spontaneous BPH dogs administered CMA for 6 months were investigated histopathologically and immunohistochemically. Male beagle dogs (5-8 years old) were divided into four experimental groups; Group 1 consisted of untreated controls. Groups 2 to 4 received CMA 0.03, 0.1, and 0.3 mg/kg/day, p.o., respectively, for 6 months. In group 1, glandular hyperplasia of the prostate was clearly detected. In groups 2 to 4, CMA produced marked atrophy of the glandular epithelium. The interacinar fibro-muscular stroma was prominent. To evaluate the frequency of apoptosis, we counted the positive cells stained by the nick end labeling method. In group 1, the apoptotic index was 0.68 ± 0.03 %. In groups 2 to 4, apoptotic indices were 10.32 ± 0.96 %, 2.05 ± 0.88 %, and 1.15 ± 0.75 %, respectively. Apoptotic cell death was mainly observed in the glandular epithelial cells. Based on our data, regression of BPH after treatment with CMA may be apoptotic cell death.