TOXIC EFFECTS OF CARBON TETRACHLORIDE ON THE PRIMARY HEPATOCYTE CULTURE FROM MONGOLIAN GERBIL (Meriones unguiculatus)

抄録

Carbon tetrachloride (CCl₄) hepatotoxicity to the primary hepatocyte culture of Mongolian gerbils was studied. Transaminase leakage from cultured cells appeared at 3h-exposure to 0.5 or 1.0mM CCl₄ remarkably increasing at 24h. With 0.5mM CCl₄, there were marked increase of lipid droplets. aggregation of swollen smooth-surfaced endoplasmic reticulums (sER), dilatation of ER lumen, metamor-phosis of mitochondria, and reduction of periodic acid-Schiff (PAS) reactivity. Cholesterol clefts were produced in ER areas at 3h-exposure, increasing in size and number with time course. These mor-phological changes due to CCl₄ were enhanced by previous treating of the culture with phenobarbital.