Journal of Toxicologic Pathology
Online ISSN : 1881-915X
Print ISSN : 0914-9198
ISSN-L : 0914-9198
IMMUNOLOCALIZATION OF GLUTATHIONE-REDUCTASE (GSSG-RD) IN THE RAT ADRENAL CORTEX
EFFECT OF HYPOPHYSECTOMY AND ACTH ADMINISTRATION
村越 正典稲田 理恵田川 正志鈴木 稔渡辺 慶一
著者情報
ジャーナル フリー

1995 年 8 巻 1 号 p. 51-57

詳細
抄録
Immunocytochemical localization of glutathione-reductase (GSSG-RD) in rat adrenal glands was studied in 1) normal untreated, 2) hypophysectomized, and 3) ACTH administered groups in order to clarify the biological significance of GSSG-RD in the adrenal cortical lipid metabolism including steroidogenesis. In the normal untreated group, GSSG-RD was immunohistochemically localized in all three zones of the adrenal cortex. In immunoelectron microscopic investigations, GSSG-RD was observed mainly in mitochondria. The hypophysectomized group showed atrophy of the zonae fasciculata and reticularis. GSSG-RD staining of the atrophic cortical zones was weakened. In immunoelectron microscopic investigations, GSSG-RD was observed not only in mitochondria but also in cytoplasmic matrix. On ACTH administration, the adrenal cortex became markedly hypertrophic, especially in the outer fasciculata. Immunohistochemically, GSSG-RD was mainly localized in the cells of those hypertrophic cortical zones. In immunoelectron microscopic investigations, GSSG-RD was observed not only in mitochondria but also in cytoplasmic matrix. These staining patterns were similar to those of glutathione-peroxidase (GSH-PO). GSH-PO reduces the lipid peroxides with concomitant oxidation of glutathione. Oxidized glutathione is reduced by GSSG-RD under the presence of NADPH. These two enzymes are known to be key enzymes of the lipid peroxides scavenging system. Based on our findings, it is strongly suggested that a very close relationship lies between the adrenal cortical GSSG-RD and steroidogenesis.
著者関連情報
© The Japanese Society of Toxicologic Pathology
前の記事 次の記事
feedback
Top