Evaluation of the toxicity of mixtures of diesel and gasoline to the marine organism mysidopsis juniae

抄録

Gasoline and Diesel consist of a wide range of components like BTEX and PAH that are known as harzard compounds to the environment and recognized as endocrine disruptors. This study evaluated the toxic effect of various concentrations of mixtures made with diesel and gasoline, in the test organism Mysidopsis juniae. In order to conduct the experiment we prepared two stock solutions of 1 liter, containing 50 ml of diesel in the first and in the second another 50 ml of gasoline. To each, 950 ml of deionized water is added to complete. The solution was stirred continuously for 24 hours. The portion of water soluble fuel was used for the preparation of experiments. We conducted a complete factorial design 3² in triplicate, using levels of 0.5%, 2.5% and 5% (volume / volume) of two fuels in reconstituted seawater with salinity of 32. Five individual organisms between the age of 3 and 5 days are placed in each flask. The organisms were fed with Artemia sp. nauplii. The methodology used included readings of the test for the observation of mortality with 24 and 48 hours after the exposure, At 24 hours, it was evident that there was total mortality of organisms at all concentrations, independent of the proportions diesel gasoline ratio. We suggest further studies to detect the minimum levels of contamination of these fuels that can cause acute toxicity, as well as kinetics and chronic toxicity which can be of use as control measure in case of environmental accidents.