主催: 日本毒性学会
The oral adminstration of five leaf extracts namely Cathranthus roseus, Datura metal, Delonix regia, Eucalyptus globules and Pongamia glabra on efficiency of total free amino acids in the hemolymph of Pericallia ricini, a serious lepidopteran pest on castor Ricinus communis, was assessed. The total amino acids showed quantitative difference in the hemolymph of normal and treated larvae. HPLC Analysis of the haemolymph of P. ricini revealed the presence of 17 aminoacids. Serine, Histidine, Glutamic acid, Aspartic acid, Arginine and Methionine exhibited quantitative decrease while Alanine, Phenylalanine, Isoleucine, Leucine showed an obvious increase after treatment of the plant extracts. Some amino acids such as Asparagine, Glutamine, Threonine, Valine, Ornithine were found in plant extract treated sample but it was absent in control insects. Along with the depletion of amino acids, there was also a drastic change in the protein profile of the hemolymph of P.ricini. It is evident that the plant extracts induced significant reduction in protein content and amino acids. The depletion in amino acids adversely affected the protein turnover in P. ricini. The depletion of aminoacids and protein due to plant extract treatment caused an upset in the balance of set patterns of growth in treated insects. It is suggested that plant origin insecticides isolated from C. roseus, D. metal, D. regia, E. globules and P. glabra may be used as an alternatives in the management of insect pest P. ricini because they will be highly economical and less hazardous to the environment.
