抄録
BACKGROUND
Rutin is a citrus flavonoid containing flavonol quercetin and disaccharide rutinose. This compound is found in a wide variety of plants including citrus fruit. It has been reported to exhibit many pharmacological activities, including antioxidant, anti-inflammatory, anti-allergy, and antitumor activities. In this study, we evaluated the hepatoprotective effects and antioxidants activities of rutin against H2O2-induced oxidative stress in HepG2 cells.
METHODS
HepG2 cells were treated with various concentrations of rutin (1-100 µM) for 3, 6 or 12 h prior to incubation with 500 µM of H2O2 for 24 h. Cell viability and ROS generation were assessed by MTT and DCFH-DA assays, respectively. Oxidative defense mechanisms were investigated by measuring glutathione levels and activities of antioxidant enzymes.
RESULTS AND DISCUSSION
The results showed that rutin at concentrations of 1-100 µM significantly improved cell viability and prevented ROS generation induced by H2O2 in HepG2 cells. The compound increased the activities of antioxidant enzymes (CAT, GPx, GR and SOD), total GSH, GSH and the GSH/GSSG ratio, but decreased GSSG. Thus, this study demonstrated that rutin possessed hepatoprotective effect against H2O2-induced oxidative stress in HepG2 cells. Attenuation of ROS production, modulation of GSH level and activation of antioxidant enzymes including CAT, GPx, GR and SOD could contribute to this effect.
