Protective effects of nintedanib against polyhexamethylene guanidine-induced pulmonary fibrosis in mice

抄録

Nintedanib (NDN), a tyrosine kinases inhibitor, has been revealed anti-tumor, anti-inflammatory, and anti-fibrotic effects in several reports. In the present study, we investigated the protective effects of NDN against polyhexamethylene guanidine phosphate (PHMG)-induced lung fibrosis in mice. The following three experimental groups were evaluated: (1) vehicle control, (2) PHMG (1.1 mg/kg), and (3) PHMG&NDN (60 mg/kg). Mice in the PHMG group exhibited increased numbers of total cells and inflammatory cells in the bronchoalveolar lavage fluid (BALF) accompanied by inflammation and fibrosis in lung tissue. In contrast. mice in the PHMG&NDN group were attenuated these lesions in lung tissue. Moreover, the inflammatory cytokines and fibrotic factors, and the activation of NLRP3 inflammasome were significantly decreased in lung tissue of PHMG&NDN group compared with that the PHMG group. These results suggest that NDN may alleviate the inflammatory response and development of pulmonary fibrosis in the lungs of PHMG-treated mice.