Analysis of in short-term bait switching test to choline-deficient, methionine-lowered, amine acid-defined diet (CDAA) induced NASH (Nonalcoholic Steatohepatitis) -like pathology in rats

Abstract

NASH is one of the most serious liver disease worldwide, but onset mechanism of NASH is still unknown. Six-week-old male F344 rats were fed a CDAA diet or a normal diet (SD) for 1 to 2 weeks. In the diet switching group, the CDAA diet was fed for 1 week, then switched to SD and fed for 1 week. Most of the changes expressed by feeding the CDAA diet for 2 weeks were ameliorated by switching the diet, however, the expression of some genes was not attenuated. By comparing this result with the previous NGS analysis obtained in 2 weeks study, it will be possible to identify the target factors involved in the progression of NASH from an early stage.