Host: The Japanese Society of Toxicology
Name : The 50th Annual Meeting of the Japanese Society of Toxicology
Date : June 19, 2023 - June 21, 2023
“Exposome” can be defined as the totality of environmental factors to which an individual in a lifetime, and these environmental factors have a greater impact on humans than genetic factors, contributing to diseases such as cancer. Although there is currently no known method to accurately identify the exposome, we believe that interactions between proteins and environmental substances are essential for environmental substances to trigger biological reactions, and we have proposed the "adduct exposome" to examine protein modifications by environmental substances. Currently, DDA (data dependent aquisition, also called shotgun analysis) is the mainstream proteomics method to comprehensively examine protein modifications, and we are attempting to elucidate the adduct exosome based on label-free quantification (LFQ) using this method. We have developed a method for proteomic analysis by adding various compounds to cells. In collaboration with Prof. Akihiro Ito and colleagues at Tokyo University of Pharmacy and Life Sciences, we added food additives to cells and searched for histone modifications in cells. They found that many organic acid compounds exhibit covalent binding to histones, which may regulate gene expression in an epigenetic manner. We will continue to investigate various compounds and protein modifications to study the relationship between compound exposure and biological reactions such as aging, addiction, and disease.
