Host: The Japanese Society of Toxicology
Name : The 50th Annual Meeting of the Japanese Society of Toxicology
Date : June 19, 2023 - June 21, 2023
We previously developed Alkyne-tag Raman Imaging (ATRI) method, in which alkyne-tagged small molecules are visualized in live cells by using Raman microscope. Alkyne tag is compact and shows a strong Raman signal in the cellular silent region where no endogenous biomolecules have Raman signals. We applied this method for the analysis of adduct-forming compounds. In addition, we succeeded in the improvement of Raman signal of alkyne tag by using Surface Enhanced Raman Scattering (SERS) on metal nanoparticles, and it realized the analysis of protein adducts and cellular uptake. Furthermore, we recently investigated Raman imaging using deuterium, which is more compact than alkyne. In this presentation, the detection of adduct-forming chemicals by Raman spectroscopy will be presented.
Reference 1) H. Yamakoshi, K. Dodo, M. Okada, J. Ando, A. Palonpon, K. Fujita, S. Kawata, M. Sodeoka, J. Am. Chem. Soc. 2011, 133, 6102. 2) H. Yamakoshi, K. Dodo, A. F. Palonpon, J. Ando, K. Fujita, S. Kawata, M. Sodeoka, J. Am. Chem. Soc. 2012, 134, 20681. 3) J. Ando, M. Asanuma, K. Dodo, H. Yamakoshi, S. Kawata, K. Fujita, M. Sodeoka, J. Am. Chem. Soc. 2016, 138, 13901. 4) K. Koike, K. Bando, J. Ando, H. Yamakoshi, N. Terayama, K. Dodo, N. I. Smith, M. Sodeoka, K. Fujita, ACS Nano, 2020, 14, 15032. 5) K. Dodo, A. Sato, Y. Tamura, S. Egoshi, K. Fujiwara, K. Oonuma, S. Nakao, N. Terayama, M. Sodeoka, Chem. Commun., 2021, 57, 2180. 6) K. Dodo, K. Fujita, M. Sodeoka, J. Am. Chem. Soc., 2022, 144, 19651.
