Information obtained from biodistribution tests conducted in non-clinical studies is important in the development of regenerative medicine products.
Some evaluation methods have been reported for detecting administered human cells, including quantitative PCR (qPCR), flow cytometry (FCM), and immunohistochemical staining (IHC). Since each product has its strengths and weaknesses, it is necessary to develop an optimal evaluation method for each product.
In a previous study, we measured human mesenchymal stem cells (hMSCs) in the blood of mice using qPCR and FCM methods. As a result, differences in hemodynamics were observed depending on the time elapsed after administration, suggesting that differences in measurement methods may have an effect.
In this study, we developed qPCR and IHC analysis methods to evaluate the distribution in mouse tissues, and compared the bio-distribution of hMSCs 30 minutes after administration.
In the qPCR method, the number of hMSC cells was calculated using the standard curve method and compared between each tissue, and 3931 to 4530 hMSC cells were detected in the lungs.
In the IHC method, immunostaining using anti-HLA (class 1 A, B, C) antibodies was performed on the lungs, liver, spleen, cerebrum and cerebellum, kidneys, and heart, and HLA-positive cells were found in the blood vessels of the lungs. It was seen here and there.
There is little knowledge of evaluating the same sample using different measurement methods, and it is hoped that the results of this study will help in selecting evaluation systems when developing regenerative medicine products.
