Copper sulfide nanoparticles were successfully prepared by laser ablation in liquid. CuS powders in deionized water were irradiated with nanosecond-pulsed laser (Nd:YAG, SHG) to prepare nanoparticles. Prepared nanoparticles were investigated by scanning electron microscopy (SEM), dynamic light scattering (DLS) and fluorospectrometer. According to the results of SEM and DLS, the primary and secondary particle size was decreased with the increase in laser fluence of laser ablation in liquid. The ratio of Cu and S of prepared nanoparticles were not changed. The absorbance of prepared copper sulfide nanoparticles in water was increased with the increase in laser fluence.